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مقاله
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Abstract
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Title:
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Anti-VEGF drugs effect RPE cells under oxidative stress
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Author(s):
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Mahdy Ranjbar
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Presentation Type:
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Oral
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Subject:
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Posterior Segment and Uveitis
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Others:
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Presenting Author:
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Name:
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Mahdy Ranjbar
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Affiliation :(optional)
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University of Lübeck
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E mail:
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mahdy.ranjbar@uksh.de
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Phone:
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0049-451-500-6879
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Mobile:
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Purpose:
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VEGF-A is induced by oxidative stress, and functions as a survival factor for various cell types, including retinal pigment epithelial (RPE) cells. Anti-vascular endothelial growth factor (VEGF) drugs have shown to be most effective in treating neovascular age-related macular degeneration (AMD), however uptake of the drugs might lead to interference with cell physiology. Herein, we evaluated the effect of the drugs on RPE cells under under oxidative stress.
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Methods:
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ARPE-19 (human RPE) cells were treated with anti-VEGF drugs in presence or absence of H2O2 as oxidative stress stimulus. After 24h cells were evaluated for various parameters.
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Results:
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All drugs inhibited extracellular levels of VEGF-A and were taken up into the RPE, resulting in significantly reduced intracellular levels of VEGF-A. When oxidative stress was applied, cell proliferation was reduced.
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Conclusion:
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Our results indicate that uptake and accumulation of anti-VEGF drugs within RPE cells affect the intracellular VEGF-A metabolism negatively, leading to a biologically relevant reduced cell survival under oxidative stress.
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Attachment:
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