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مقاله
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Abstract
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Title:
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Amniotic Membrane Extract Eye Drop (AMEED) promotes human limbal stem cell proliferation and induces Corneal Epithelium healing in Rabbit model
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Author(s):
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Farhad Nejat, Niloufar Shayan Asl , Marzieh Ebrahimi, Khosrow Jadidi , Parvaneh Mohammadi, Abdolhossein Nekoukar, Saeed Hesam
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Presentation Type:
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Oral
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Subject:
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Cornea and Anterior Segment
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Others:
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Presenting Author:
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Name:
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Farhad Nejat
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Affiliation :(optional)
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Basir eye health research center. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
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E mail:
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fanejat@yahoo.com
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Phone:
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96662102
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Mobile:
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09124014938
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Purpose:
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Evaluation of the effect of human amniotic membrane extract eye drop (AMEED) on human LSCs proliferation and corneal healing in rabbit model
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Methods:
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Human amniotic membrane were extracted from healthy donor amnion tissues and were added to the human LSCs cultures for 14 days to assess effective and cytotoxic dose. ABCG2 as LSC and K3, K12 and K19 as corneal differentiation specific genes were assessed by using Real Time PCR. The corneal epithelium of 10 rabbits was mechanically removed; the left eye of each rabbit was treated with 1 mg/ml of AMEED (2 hours and 6 hours, respectively) and the right eye was treated only with antibiotic. The corneal healing was observed daily, scored by ophthalmologists, and was biopsied for pathology.
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Results:
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AMEED at concentration of 0.1-1mg/ml induced LSC proliferation, upregulated ABCG2 and downregulated K3 in comparison to cells grown in the presence of 10% fetal bovine serum (FBS). Interestingly, in rabbits which were treated with AMEED, the time of epithelium healing decreased to 2-3 days with lower Edema, chemosis and injection in comparison to rabbits in control group. No symptoms of pathologic abnormalities were observed in both groups.
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Conclusion:
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The results indicate that AMEED increases the LSCs proliferation in vitro and accelerates the corneal epithelium healing in vivo without any adverse effect. Therefore, it can be easily applied as supplement in limbal cell expansion for cellular therapy
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Attachment:
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